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polyclonal anti orp8 antibody (Boster Bio)

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Boster Bio polyclonal anti orp8 antibody

Knockdown of Atg8 and <t>Orp8</t> blocked lipophagy. A , interference efficiency was detected by Western blotting. B , the colocalization of lysosomes and LDs in fat body cells after dsAtg8 injection. Lysosomes were stained with Lyso-Tracker ( red ), and LDs were stained with BODIPY ( green ). Yellow indicates examples of Lyso-Tracker-positive structures containing LDs. The ruler represents 50 μm. Bi , statistical analysis of autolysosome numbers in ( B ). C , the levels of triglycerides in the fat body after Atg8 knockdown. D , the location of ORP8 in fat body cells. Red fluorescence represents ORP8; green fluorescence represents LDs. The scale bar is 50 μm. E , the specificity of the ORP8 antibody in the fat body was analyzed by Western blotting. F , the expression of ORP8 was analyzed by Western blotting. G and Gi , the colocalization of LDs and lysosomes was detected after Orp8 knockdown. The ruler represents 50 μm. H , the levels of triglycerides in the fat body after Orp8 knockdown. I , phenotypes after dsOrp8 injection. Bars represent 1 cm. dsGfp was used as a control. J , statistical analysis of the phenotypes in ( I ). The pupation time was sixth instar 0 h larvae to pupae. K , the average weight of pupae and pupal phenotypes. L , percentage of different phenotypes from pupae to adults. The bars indicate the mean ± SD. p values and asterisks indicate differences by two-tailed Student's t test (∗ p < 0.05, ∗∗ p < 0.01). The different lowercase letters indicate significant differences in ANOVA ( p < 0.05). LD, lipid droplet; ORP8, oxysterol-binding protein-related protein 8.

Polyclonal Anti Orp8 Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyclonal+anti+orp8+antibody/pmc11835591-518-0-8?v=Boster+Bio
Average 94 stars, based on 1 article reviews

polyclonal anti orp8 antibody - by Bioz Stars, 2026-08

94/100 stars

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1) Product Images from "The steroid hormone 20-hydroxyecdysone induces lipophagy via the brain-adipose tissue axis by promoting the adipokinetic hormone pathway"

Article Title: The steroid hormone 20-hydroxyecdysone induces lipophagy via the brain-adipose tissue axis by promoting the adipokinetic hormone pathway

Journal: The Journal of Biological Chemistry

doi: 10.1016/j.jbc.2025.108179

Knockdown of Atg8 and Orp8 blocked lipophagy. A , interference efficiency was detected by Western blotting. B , the colocalization of lysosomes and LDs in fat body cells after dsAtg8 injection. Lysosomes were stained with Lyso-Tracker ( red ), and LDs were stained with BODIPY ( green ). Yellow indicates examples of Lyso-Tracker-positive structures containing LDs. The ruler represents 50 μm. Bi , statistical analysis of autolysosome numbers in ( B ). C , the levels of triglycerides in the fat body after Atg8 knockdown. D , the location of ORP8 in fat body cells. Red fluorescence represents ORP8; green fluorescence represents LDs. The scale bar is 50 μm. E , the specificity of the ORP8 antibody in the fat body was analyzed by Western blotting. F , the expression of ORP8 was analyzed by Western blotting. G and Gi , the colocalization of LDs and lysosomes was detected after Orp8 knockdown. The ruler represents 50 μm. H , the levels of triglycerides in the fat body after Orp8 knockdown. I , phenotypes after dsOrp8 injection. Bars represent 1 cm. dsGfp was used as a control. J , statistical analysis of the phenotypes in ( I ). The pupation time was sixth instar 0 h larvae to pupae. K , the average weight of pupae and pupal phenotypes. L , percentage of different phenotypes from pupae to adults. The bars indicate the mean ± SD. p values and asterisks indicate differences by two-tailed Student's t test (∗ p < 0.05, ∗∗ p < 0.01). The different lowercase letters indicate significant differences in ANOVA ( p < 0.05). LD, lipid droplet; ORP8, oxysterol-binding protein-related protein 8.

Figure Legend Snippet: Knockdown of Atg8 and Orp8 blocked lipophagy. A , interference efficiency was detected by Western blotting. B , the colocalization of lysosomes and LDs in fat body cells after dsAtg8 injection. Lysosomes were stained with Lyso-Tracker ( red ), and LDs were stained with BODIPY ( green ). Yellow indicates examples of Lyso-Tracker-positive structures containing LDs. The ruler represents 50 μm. Bi , statistical analysis of autolysosome numbers in ( B ). C , the levels of triglycerides in the fat body after Atg8 knockdown. D , the location of ORP8 in fat body cells. Red fluorescence represents ORP8; green fluorescence represents LDs. The scale bar is 50 μm. E , the specificity of the ORP8 antibody in the fat body was analyzed by Western blotting. F , the expression of ORP8 was analyzed by Western blotting. G and Gi , the colocalization of LDs and lysosomes was detected after Orp8 knockdown. The ruler represents 50 μm. H , the levels of triglycerides in the fat body after Orp8 knockdown. I , phenotypes after dsOrp8 injection. Bars represent 1 cm. dsGfp was used as a control. J , statistical analysis of the phenotypes in ( I ). The pupation time was sixth instar 0 h larvae to pupae. K , the average weight of pupae and pupal phenotypes. L , percentage of different phenotypes from pupae to adults. The bars indicate the mean ± SD. p values and asterisks indicate differences by two-tailed Student's t test (∗ p < 0.05, ∗∗ p < 0.01). The different lowercase letters indicate significant differences in ANOVA ( p < 0.05). LD, lipid droplet; ORP8, oxysterol-binding protein-related protein 8.

Techniques Used: Knockdown, Western Blot, Injection, Staining, Fluorescence, Expressing, Control, Two Tailed Test, Binding Assay

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Journal: The Journal of Biological Chemistry

Article Title: The steroid hormone 20-hydroxyecdysone induces lipophagy via the brain-adipose tissue axis by promoting the adipokinetic hormone pathway

doi: 10.1016/j.jbc.2025.108179

Figure Lengend Snippet: Knockdown of Atg8 and Orp8 blocked lipophagy. A , interference efficiency was detected by Western blotting. B , the colocalization of lysosomes and LDs in fat body cells after dsAtg8 injection. Lysosomes were stained with Lyso-Tracker ( red ), and LDs were stained with BODIPY ( green ). Yellow indicates examples of Lyso-Tracker-positive structures containing LDs. The ruler represents 50 μm. Bi , statistical analysis of autolysosome numbers in ( B ). C , the levels of triglycerides in the fat body after Atg8 knockdown. D , the location of ORP8 in fat body cells. Red fluorescence represents ORP8; green fluorescence represents LDs. The scale bar is 50 μm. E , the specificity of the ORP8 antibody in the fat body was analyzed by Western blotting. F , the expression of ORP8 was analyzed by Western blotting. G and Gi , the colocalization of LDs and lysosomes was detected after Orp8 knockdown. The ruler represents 50 μm. H , the levels of triglycerides in the fat body after Orp8 knockdown. I , phenotypes after dsOrp8 injection. Bars represent 1 cm. dsGfp was used as a control. J , statistical analysis of the phenotypes in ( I ). The pupation time was sixth instar 0 h larvae to pupae. K , the average weight of pupae and pupal phenotypes. L , percentage of different phenotypes from pupae to adults. The bars indicate the mean ± SD. p values and asterisks indicate differences by two-tailed Student's t test (∗ p < 0.05, ∗∗ p < 0.01). The different lowercase letters indicate significant differences in ANOVA ( p < 0.05). LD, lipid droplet; ORP8, oxysterol-binding protein-related protein 8.

Article Snippet: Polyclonal anti-ORP8 antibody (anti-OSBPL8 antibody) was obtained from Boster Biological Technology.

Techniques: Knockdown, Western Blot, Injection, Staining, Fluorescence, Expressing, Control, Two Tailed Test, Binding Assay

( A ) Representative images of mitochondrial morphology (labeled by mito-BFP, gray) of U-2 OS cells transfected with empty vector (EV) (mCherry-N1, magenta) and with either siCTRL and mNG-C1 EV (green, n=28 cells, left); OSBPL8 siRNA and mNG-C1 EV (green, n=21 cells, middle); or OSBPL8 siRNA and siRNA-resistant mNG-ORP8 (green, n=41 cells, right). ( B ) Representative images of mitochondrial morphology (labeled by mito-BFP, gray) of U-2 OS cells transfected with ABHD16A-mCherry overexpression (OE; magenta) and either siCTRL and mNG-C1 EV (green, n=35 cells, left); OSBPL8 siRNA with mNG-C1 EV (green, n=37 cells, middle); or OSBPL8 siRNA with siRNA-resistant mNG-ORP8 (green, n=37 cells, right). ( C ) Representative images of mitochondrial morphology (labeled by mito-BFP, gray) of U-2 OS cells transfected with either EV (mCherry-N1, magenta) and siCTRL (n=28 cells); EV and VPS13A/D siRNA (n=30 cells); with ABHD16A-mCherry OE (magenta) and siCTRL (n=36 cells); or with ABHD16A-mCherry OE (magenta) and VPS13A/D siRNA (n=29 cells). ( D ) Quantification of mean mitochondrial size (area per mitochondrion in µm 2 ) within a 15×15 µm region of interest (ROI) from ( A ) and ( B ). ( E ) Quantification of mean mitochondrial size (area per mitochondrion in µm 2 ) within a 15×15 µm ROI from ( C ). ( F ) Representative immunoblot shows efficiency of depletion in U-2 OS cells from ( A ) and ( B ) treated with control siRNA or OSBPL8 siRNA and rescued with siRNA-resistant mNG-ORP8. GAPDH serves as a loading control. Asterisk indicates non-specific band. ( G ) Representative immunoblot shows efficiency of depletion in U-2 OS cells from ( C ) treated with control siRNA or VPS13A/D siRNA. GAPDH serves as a loading control. All data were taken from three biological replicates; statistical significance was calculated by one-way ANOVA. n.s., not significant; ***p≤0.001. Scale bar = 5 µm. See , , , , , , , , . Figure 7—source data 1. Related to . Figure 7—source data 2. Related to . Figure 7—source data 3. Related to . Figure 7—source data 4. Related to . Figure 7—source data 5. Related to . Figure 7—source data 6. Related to . Figure 7—source data 7. Related to . Figure 7—source data 8. Related to . Figure 7—source data 9. Related to .

Journal: eLife

Article Title: An ER phospholipid hydrolase drives ER-associated mitochondrial constriction for fission and fusion

doi: 10.7554/eLife.84279

Figure Lengend Snippet: ( A ) Representative images of mitochondrial morphology (labeled by mito-BFP, gray) of U-2 OS cells transfected with empty vector (EV) (mCherry-N1, magenta) and with either siCTRL and mNG-C1 EV (green, n=28 cells, left); OSBPL8 siRNA and mNG-C1 EV (green, n=21 cells, middle); or OSBPL8 siRNA and siRNA-resistant mNG-ORP8 (green, n=41 cells, right). ( B ) Representative images of mitochondrial morphology (labeled by mito-BFP, gray) of U-2 OS cells transfected with ABHD16A-mCherry overexpression (OE; magenta) and either siCTRL and mNG-C1 EV (green, n=35 cells, left); OSBPL8 siRNA with mNG-C1 EV (green, n=37 cells, middle); or OSBPL8 siRNA with siRNA-resistant mNG-ORP8 (green, n=37 cells, right). ( C ) Representative images of mitochondrial morphology (labeled by mito-BFP, gray) of U-2 OS cells transfected with either EV (mCherry-N1, magenta) and siCTRL (n=28 cells); EV and VPS13A/D siRNA (n=30 cells); with ABHD16A-mCherry OE (magenta) and siCTRL (n=36 cells); or with ABHD16A-mCherry OE (magenta) and VPS13A/D siRNA (n=29 cells). ( D ) Quantification of mean mitochondrial size (area per mitochondrion in µm 2 ) within a 15×15 µm region of interest (ROI) from ( A ) and ( B ). ( E ) Quantification of mean mitochondrial size (area per mitochondrion in µm 2 ) within a 15×15 µm ROI from ( C ). ( F ) Representative immunoblot shows efficiency of depletion in U-2 OS cells from ( A ) and ( B ) treated with control siRNA or OSBPL8 siRNA and rescued with siRNA-resistant mNG-ORP8. GAPDH serves as a loading control. Asterisk indicates non-specific band. ( G ) Representative immunoblot shows efficiency of depletion in U-2 OS cells from ( C ) treated with control siRNA or VPS13A/D siRNA. GAPDH serves as a loading control. All data were taken from three biological replicates; statistical significance was calculated by one-way ANOVA. n.s., not significant; ***p≤0.001. Scale bar = 5 µm. See , , , , , , , , . Figure 7—source data 1. Related to . Figure 7—source data 2. Related to . Figure 7—source data 3. Related to . Figure 7—source data 4. Related to . Figure 7—source data 5. Related to . Figure 7—source data 6. Related to . Figure 7—source data 7. Related to . Figure 7—source data 8. Related to . Figure 7—source data 9. Related to .

Article Snippet: Rabbit anti-ORP8 polyclonal antibody (GeneTex, Cat# GTX121273) was used at 1:1000 for western blot.

Techniques: Labeling, Transfection, Plasmid Preparation, Over Expression, Western Blot, Control

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1) Product Images from "The steroid hormone 20-hydroxyecdysone induces lipophagy via the brain-adipose tissue axis by promoting the adipokinetic hormone pathway"

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